UV-Vis Spectrophotometer: Quantifying Molecular Interactions with Nanogram Accuracy

Applications in Detail

• Pharmaceutical QC: Quantify API (Active Pharmaceutical Ingredient) concentration per USP <857>, ensuring compliance with dissolution testing protocols.

• Environmental Analysis: Detect heavy metals (e.g., lead at 283.3 nm) in water samples via EPA Method 6010.

• CRISPR Research: Measure sgRNA purity (A260/A280 >1.8) for gene-editing applications.

Technical Innovations

• Optical Path: Czerny-Turner monochromator with 1200 lines/mm grating, achieving 0.1 nm resolution (190–1100 nm range).

• Detector Technology: Thermoelectrically cooled CCD array reduces dark noise to <0.0003 AU.

• Software Suite: LabSolutions™ supports GLP compliance, including automatic cuvette pathlength correction (1–100 mm).

Validation & Standards

• NIST-traceable wavelength calibration (±0.1 nm) and photometric accuracy (±0.002 AU).

• Meets ISO 17025 requirements for analytical instrument qualification (AIQ).

User Workflow Example
A contract lab streamlined antibiotic potency testing by pre-programming methods for ciprofloxacin (λmax=277 nm) and amoxicillin (λmax=229 nm), reducing analysis time by 40%.